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Monolith uses MicroScale Thermophoresis (MST) technology to quantify binding events. One partner is typically labeled with a fluorescent dye and becomes an extremely sensitive reporter for all molecular interactions. These events, weak or strong, cause a change in MST signal that is easily detected The experimental setup for detecting MicroScale Thermophoresis is as follows: In devices of the Monolith-series (e.g. in the Monolith NT.115, Fig. 1 A), thermophoresis is induced and detected in small glass capillaries which contain a solution of fluorescent molecules Diese sogenannte Thermophorese hängt von Größe, Ladung und Hydrathülle des Moleküls ab, die sich bei Bindung eines Liganden verändern. Für die Messung wird das zu untersuchende Molekül fluoreszenz-markiert. Die Thermophorese wird nach lokaler Erhitzung durch einen Infrarotlaser optisch erfasst

MST setup and experiments

Nanotemper Monolith thermophoresis instruments (one for intrinsic fluorescence reporters and one for extrinsic reporters) Microscale Thermophoresis (MST) is a powerful method to quantify biomolecular interactions MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge, or size

The Monolith NT.115 instrument has to be installed in a way that it does not hinder the access to the external power supply and its power plug. CAUTIONThe weight of the Monolith NT.115 instrument is approx. 24 kg, do not move the instrument alone (two persons required for transport) Microscale Thermophoresis can monitor the binding of single ions (40Da) or small molecules (300Da) to a target as well as the binding of ribosomes (2.5MDa). Microscale Thermophoresis is easy to handle and allows to measure the binding of biomolecules as well as the activity of enzymes

This manual is your guide for using the Monolith NT.115 and doing MicroScale Thermophoresis (MST) measurements. It instructs first-time users on how to use the instrument, and serves as a reference for experienced users Thermophoresis, the movement of the molecule in the temperature gradient, depends on three parameters that typically change upon interaction. Thus, the overall MST signal is plotted against the ligand concentration to obtain a dose-response curve, from which the binding affinity can be deduced PROTEIN QUALITY. Tycho overview Identify protein quality Tycho applications Explore ways to use Tycho Tycho for SPR Increase your SPR productivity Get to know Tycho See Tycho and how to run an assay Tycho purchase packages Get an instant quote to buy Tycho Tycho subscription plans Get Tycho only for the time you nee MicroScale Thermophoresis or MST is a technique that allows you to obtain binding constants for virtually any type of biomolecular interaction in a close-to-native environment. MST can overcome.

MST Systems for Binding Affinity & Protein Interaction

  1. Thermophoresis (also thermomigration, thermodiffusion, the Soret effect, or the Ludwig-Soret effect) is a phenomenon observed in mixtures of mobile particles where the different particle types exhibit different responses to the force of a temperature gradient
  2. The dye is suited for the RED channel of Monolith NT.115 Green/Red and Blue/Red instruments. Applications: Kit for 1h labeling and purification of 4x 100 µg protein with the dye NT-647 ; effective removal of free, unreacted dye; achieve a 1:1 ratio of labeled protein to dye; the fluorescent dyes are widely tested with Microscale Thermophoresis (MST) Type: 1h labeling and purification; Read.
  3. Thermophoresis is the movement of suspended particles through a fluid under the influence of an applied thermal gradient [ 60 ]
  4. The Monolith NT.115 combines the versatility to use of a broad range of fluorophores and fluorescent proteins with all benefits of the MicroScale Thermophoresis technology. The instrument is equipped with one Nano detector that has two different fluorescence channels. The Monolith NT.115 allows measuring every interaction type - ranging from the formation of huge protein complexes to the.
  5. Thermophoresis of DNA. Share; The thermophoretic movement of DNA, stained with Sybr Gold, is monitored with a fluorescence micrsocope. Using scanning mirrors we moved an IR-Laser along the letters LMU. The aqueos solution absorbs the IR-Laser radiation and thus the temperature at these letters is locally heated up by 2K. The stained DNA moves from hot to cold, thus the concentration of.
  6. Monolith NT.115 instrument has to be installed in a way that it does not hinder the access to the external power supply and its power plug. CAUTION The weight of the Monolith NT.115 instrument is approx. 22 kg, do not move the instrument alone (two persons required for transport). If you move the instrument alone i
  7. Microscale Thermophoresis Technology (MST) is based on a physical principle used for the first time in biomolecule analytics

MicroScale Thermophoresis: Interaction analysis and beyond

  1. Microscale Thermophoresis (MST) Instrument Our MST instrument is a Nano-BLUE/RED Monolith NT.115 from NanoTemper Technologies, equipped with two LED-filter combinations, blue (excitation 450-480 nm, emission 515-570 nm) and red (excitation 600-645 nm, emission 670-730 nm)
  2. By combining the precision of fluorescence detection with the flexibility and sensitivity of thermophoresis, Monolith instruments provide a flexible, robust and highly versatile platform for measuring molecular interactions. The fully-automated Monolith NT.Automated is designed for easy handling and high-throughput applications
  3. e binding affinity using microscale thermophoresis (MST) MST is a powerful technique that is used to quantify biomolecular interactions such as binding affinity, protein folding, thermodynamics, and enzyme kinetics. This highly sensitive technology allows for precise quantification of these molecular events independent of their size or the.
  4. For details refer to the Concentration Finder- Tool in the Monolith Software. You can use this tool in order to simulate the binding event. It will help you to choose an optimal concentration range of the unlabeled molecule. Microscale Thermophoresis is a method that uses very small quantities and volumes of material
  5. Micro-scale thermophoresis (MST) is a powerful technique to quantify molecular interactions. During MST experiments the movement of molecules along a microscopic temperature gradient is measured. This motion is dependent on size, charge and hydration shell and changes when molecules interact. Molecular motion is monitored either via a fluorescent dye or protein (Monolith NT.115) or via.

The Monolith NT.115 measures biomolecular interactions via MicroScale Thermophoresis (MST). It quantifies biomolecular interactions in an easy, rapid and accurate fashion. During an MST experiment, molecules move along a microscopic temperature gradient which is induced by an infrared laser Microscale Thermophoresis. Messung der Thermophorese eines fluoreszenzmarkierten Biomoleküls. Die normierte Fluoreszenz im erwärmten Laser-Spot ist gegen die Zeit aufgetragen. Der IR-Laser wird zum Zeitpunkt t=5s eingeschaltet und induziert eine messbare Änderung der Fluoreszenz. Die gemessene Abnahme der Fluoreszenz wird durch zwei Effekte, die sich in ihrer Relaxationszeit unterscheiden.

Monolith NT.115 Getting Started About MST MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge or size. Since one or all of these parameters changes with virtually every. Monolith - Microscale thermophoresis binding affinity (MST) 0.00. MicroScale Thermophoresis determines binding affinities in solution. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. Time and cost effectiveness . fast measurement: Kd in 10 min. low sample consumption: minimal concentration (pM/nM) and. Instruments for Microscale Thermophoresis . Monolith NT.115 Monolith NT.115. Pico Monolith NT.LabelFree broad application range from ions to ribosomes buffer independency even serum or cell lysate purification free fluorescent fusion proteins dynamic range 1 nM to mM high sensitivity limit of detection at 50 pM low sample consumption pM concentrations better resolution for high affinity.

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Microscale Thermophoresis Grade Kit for 1h labeling of proteins with the BLUE fluorescent dye NT-495 for use in Microscale Thermophoresis CONTENT AND STORAGE Monolith NT™ Protein Labeling Kits are shipped on at room temperature . Store at 4°C upon arrival. Each kit contains material sufficient for 4 protein labeling reactions. Depending on the amount of protein used, enough material for. Microscale Thermophoresis: Nanotemper Monolith NT.115; Multiangle Light Scattering: WYATT Minidawn Treos And Optilab Rex; Circular Dichroism and Fluorescence: Jasco J-1500; Macromolecular Crystallization; Mass Spectrometry. Instrument List; Instrument Rates; Sample Submission; Biochemistry & Cell Biology. Cell-based Assays: GE Cytell Automated. Über 80% neue Produkte zum Festpreis; Das ist das neue eBay. Finde ‪Monolithic‬! Schau Dir Angebote von ‪Monolithic‬ auf eBay an. Kauf Bunter

Nanotemper Monolith Thermophoresis : Core Facilities

Microscale thermophoresis (MST) is an emerging technique that has been broadly applied to investigate biomolecular interaction of a variety of drug targets. MST detects the directed movement of fluorescent molecules in microscopic tempera-ture gradients in microliter-volume capillaries to quantify interaction affinities4,5 (Suppl. Fig. 1). Each molecule has dis-tinct thermophoretic properties. Thermophoresis is dominated by buffer dependence. To determine the best formulation for analysis of the interaction between a protein and its ligand, several lysis buffers and several buffers for. This chapter describes the use of MicroScale Thermophoresis (MST) for the analysis and quantification of biomolecular interactions and shows how the unique properties of MST can be used to rapidly generate binding data with high‐precision and information content while minimizing time and sample consumption. It also discusses application examples, MST‐specific benefits, and the integration. MicroScale Thermophoresis The Monolith NT.115 Dinorah Leyva, Ph.D. NanoTemper Technologies Inc. 395 Oyster Point Blvd. Suite 135, South San Francisco, CA www.nanotemper-technologies.com. 1856: Carl Ludwig 1879: Charles Soret 2008 2010: Monolith NT.115 2012: Monolith NT.LabelFree Stefan Duhr Philipp Baaske Fast thermo-optical particle characterisation PCT Patent 2008/061706 2013: Monolith NT. If so, you may want to try using MicroScale Thermophoresis (MST) to quantify your molecular interactions! As part of a UW2020 instrumentation grant, the UW Carbone Cancer Center's Drug Development Core (DDC) Medicinal Chemistry Center has recently purchased Nanotemper Technologies' Monolith NT.115 pico instrument. This new technology is both general (capable of measuring small molecule.

MicroScale Thermophoresis (MST) Center for

Monolith NT.115, Fig. 1 A), thermophoresis is induced and detected. in small glass capillaries which contain a solution of fluorescent. molecules. An infrared (IR) laser with an emission. The Complete Monotub Tek Cultivation Walk-through - The Easiest Way to Grow Mushrooms Indoors! - Duration: 36:30. North Spore Recommended for yo To monitor the thermophoresis of biomolecules, the Monolith instruments commercialized by NanoTemper use an infrared (IR) laser, which heats an aqueous sample loaded in a thin glass capillary. The laser focus produces a localized microscopic temperature gradient with a heat range of 2-6 °C, and ligand-target protein complexes demonstrate a different movement velocity through the.

The thermophoresis signal is plotted against the ligand concentration to obtain a dose-response curve, from which the binding affinity can be deduced. The dynamic affinity range covers the pM to mM range, which is comparable to SPR measurements. MST requires a small amount of sample and the technique is relatively easy to use. [b] However, the requirement to use hydrophobic fluorescent labels. Monolith: The Efficient Performer Die MicroScale Thermophoresis (MST) Technologie ist eine einzigartige Methode, Bindungsaffinitäten auf einfache, schnelle und präzise Weise zu untersuchen. Vier Instrumente der Monolith-Serie wurden seit 2010 auf den Markt gebracht. Prometheus: The Stability Expert 2015 wurde mit dem Prometheus NT.48 eine neue Produktlinie eingeführt. Diese nutzt nanoDSF. Monolith NT.115, Fig. 1A), thermophoresis is induced and detected in small glass capillaries which contain a solution of fluorescent molecules. An infrared (IR) laser with an emission wavelength of 1480 nm is focused through an objective into the capillary to pro-duce a microscopic temperature gradient spanning 2-6 C in a vol- ume with a diameter of 50 lm. While inducing the temperature. Microscale thermophoresis (MST) is an emerging technique that has been broadly applied to investigate biomolecular interaction of a variety of drug targets. MST detects the directed movement of fluorescent molecules in microscopic temperature gradients in microliter-volume capillaries to quantify interaction affinities 4,5 (Suppl. Fig. 1). Each.

  1. Thermophoresis, the motion of molecules in temperature fields, is very sensitive to changes in size, charge, and solvation shell of a molecule and thus suited for bioanalytics. This review focuses on the theoretical background of MST and gives a detailed overview on various applica-tions to demonstrate the broad applicability. Experiments range from the quantification of the affinity of low.
  2. After >2 h incubation, the melanin-ligand complexes were mixed gently with a pipette to ensure melanin solution homogeneity and then transferred into capillaries (Monolith NT.115 Premium capillaries, #MOK025, NanoTemper Technologies GmbH, München, Germany). The samples were analyzed with a Monolith NT.115 pico device using MO.Control Software (both from NanoTemper Technologies GmbH.
  3. Monolith uses MicroScale Thermophoresis (MST) technology to quantify binding events. One partner is typically labeled with a fluorescent dye and becomes an extremely sensitive reporter for all molecular interactions. These events, weak or strong, cause a change in MST signal that is easily detected. The results are automatically translated into precise and quantitative In this study, we.

Video: Microscale thermophoresis - Wikipedi

MicroScale Thermophoresis (MST) is a technique complementary to SPR that is used to measure biomolecular interactions. It is based on thermophoresis, the directed movement of molecules in a temperature gradient, which strongly depends on a variety of molecular properties such as size, charge, hydration shell or conformation. Our Monolith NT.115 Blue/Red thermphoresis detector features. Thermophoresis is highly sensitive to all types of binding-induced changes of mol. properties, be it in size, charge, hydration shell or conformation. In an all-optical approach, an IR laser is used for local heating, and mol. mobility in the temp. gradient is analyzed via fluorescence. In std. MST one binding partner is fluorescently labeled. However, MST can also be performed label-free by. Das Unternehmen hat eine völlig neue Technologie zur Messung von biomolekularen Interaktionen in großer Bandbreite entwickelt, die Microscale Thermophoresis (MST). Das Messgerät Monolith NT.115 wird weltweit in der universitären Forschung sowie in Pharma- und Biotechnologie eingesetzt. Es erleichtert z.B. die Medikamentenentwicklung, weil.

Protein Characterization Methods - Protein Quantification

Bench Tip Video: Measuring Interactions Using Microscale

Thermophoresis - Wikipedi

Microscale Thermophoresis is a powerful method to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. By combining the precision of fluorescence detection with the flexibility and sensitivity of thermophoresis, MST provides a flexible, robust and fast way to measure. MicroScale Thermophoresis Monolith NT.115 (MST) Biacore X100; MicroCal iTC 200; Researchers and students can use the machines independently after an introduction. The introduction is given by Lisha and is obligatory for all new users. The reservation system works electronically via a google calendar. For first use please contact Lisha by e-mail. molecules. NanoTemper provides three Monolith series instruments based on MST: Monolith NT.115 and NT.115Pico, which measure thermophoresis of fluorescently labeled molecules. Monolith NT.LabelFree, which measures thermophoresis by intrinsic tryptophan fluorescence. Epigenetics NanoTemper´s MST technology has the following key benefits

To demonstrate the performance of Microscale Thermophoresis, we would like to invite you to analyze your samples on the Monolith NT.115 and the Monolith NT.LabelFree instruments. We also offer the possibility to label any protein in 30 minuteswith during our workshop day. For more information please contact david.witte@nanotemper.d NanoTemper Technologies is deeply committed to the best customer experience. Central to this is a strong focus on enabling researchers to easily, efficiently, a... nd accurately perform protein characterization. With a broad offering of systems, software and consumables for evaluating binding affinities and protein stability, scientists in pharmaceutical, biotech or academic labs will find an. Samples of ∼10 μL were loaded into capillaries and inserted into the MST instrument loading tray (Monolith NT.115). The thermophoresis experiments were performed using 40% MST power and 80% LED power at 25°C. T6P (50 µM) was included to test its effect on the associations of KIN10 with ATP or GRIK1. Protein Phosphorylation Assay . The in vitro protein phosphorylation assay was performed. The slower thermophoresis refers to the movement of molecules in the temperature field, offering information on the overall structure of the formed complex. The default type of data analysis employs the Thermophoresis + T-Jump setting, exploiting both phenomena to determine the binding parameters. In case the two phases have opposite directions, it is recommended to analyze the phases.

Microscale Thermophoresis NT

MicroScale Thermophoresis: Interactions and beyond Join our MST Workshop! Dr. Heide Marie Roth NanoTemper Technologies GmbH heidemarie.roth@nanotemper.de 10 -11th July 2014 Rudolf Virchow Center, Seminar Room D15.01.002-004 MicroScale Thermophoresis Technology Agenda Day 1: Seminar: MicroScale Thermophoresis technology and applications Guest speaker's talk Coffee break Seminar: Assay setup. Microscale Thermophoresis and Simple Western P4EU Meeting November 11-12, 2013. Structural Biology Facility •Campus Science Support Facilities (CSF) •Group of 10 core facilities •Serve 4 different research institutes •Campus Vienna Biocenter (VBC) •MFPL (Max Perutz Labs, Uni-Vienna) •IMP (Research Institute of Molecular Pathology) •IMBA (Institute of Molecular Biotechnology. Learn about the basics of MicroScale Thermophoresis (MST) and nanoDSF, the advanced Differential Scanning Fluorimetry (DSF) technology; Attend expert talks from invited, experienced scientists in the MST field; Use our advanced instruments Monolith and Prometheus NT.48 to measure biomolecular interactions and/or protein stability with your own samples; Discuss your experiments and data with. Nanotemper Monolith Nt, supplied by NanoTemper Technologies, used in various techniques. Bioz Stars score: 92/100, based on 791 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and mor NanoTemper's Monolith is nominated in the category B Biotechnology and Life Sciences of the G.I.T. InnovationsAward 2012. Monolith uses the new biophysical method Microscale Thermophoresis (MST) t..

Microscale Thermophoresis (MST) Protein Labeling Kits

Purchase of a NanoTemper Monolith NT.115 from NanoTemper Technologies is proposed. Microscale Thermophoresis (MST) is a relatively new technology that due to extreme ease of use and measurement quality is rapidly becoming a preferred method to determine affinities. MST measures binding affinities by measuring the motion of molecules along. Thermophoresis, the motion of molecules in temperature fields, is very sensitive to changes in size, charge, and solvation shell of a molecule and thus suited for bioanalytics. This review focuses on the theoretical background of MST and gives a detailed overview on various applications to demonstrate the broad applicability. Experiments range from the quantification of the affinity of low. Monolith NT.115 pico Automated liquid handler Labcyte Echo 525 Monolith NT.115 Monolith NT.Automated Monolith NT.LabelFree thermophoresis. Reagents. HEPES buffer solution Polysorbate 20 Tris Buffered Saline. Species. Human Mouse Rat. Microscale Thermophoresis available from. 2bind GmbH Regensburg, Germany. 6 Microscale Thermophoresis Orders . 9 Total Orders. 100 % Positive. 0 Endorsements.

Thermophoresis - an overview ScienceDirect Topic

Fig. 1 MicroScale Thermophoresis binding assays: (a) Schematic representation of the technical setup of a Monolith™ instrument used to monitor MST. (b) Plot of fluorescence intensity changes over time throughout a typical MST experiment. The schematic representations depict movement of fluorescent molecules through the temperature gradient At the European Lead Factory, a microscale thermophoresis platform from NanoTemper Technologies is helping to characterize the hits identified via high-throughput screening Thermophoresis is the phenomenon by which sub-micron particles suspended in a gas move in the direction of decreasing temperature. It occurs naturally in automotive catalysts and is responsible for improved particle deposition during transient operation when a hot, particle-laden exhaust gas flows through a cool monolith. Although the time-averaged effects of thermophoresis are expected to be. Microscale Thermophoresis (MST) is a technique that is used to quantitatively measure binding using changes in thermophoretic mobility as an indicator. The instrument will heat one spot in the cell causing molecules to diffuse across the temperature gradient (thermophoresis) and will measure the distribution of molecules using fluorescence. If two molecules in solution interact with one. thermophoresis (MST) using the MonolithTM NT.115 instrument (NanoTemper). We have used the protocol to determine the binding affinities between three different flavodoxin reductases (FNRs) and a flavodoxin-like protein, NrdI, from Bacillus cereus (Lofstad et al., 2016). NrdI is essential in the activation of the manganese-bound form of the class Ib ribonucleotide reductase (RNR) system. RNRs.

Quantum Design Taiwan - Products - MicroScale

MicroScale Thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here, we describe the application of MST in quantifying two CBM-carbohydrate interactions, a CBM3a toward cellulose nanocrystals and a CBM4. This ohenomenon is called thermophoresis. The Bioanalytics core facility is equipped with a Monolith NT.115 device (NanoTemper) Microscale-Thermophoresis device that is based on detection of fluorescent proteins or dyes. Therefore, affinities of biomolecular interactions of dye-labelled proteins or DNA e.g. with any non-labelled compound and can easily be measured in solution in any buffer or. Accelerating Lead Optimization to Clinical Application Using Microscale Thermophoresis to Quantify Molecular Interactions This project supports the purchase of a Monolith NT.115pico instrument for use in analyzing molecular interactions. UW-Madison has an urgent need for a general and sensitive method to detect and quantify molecular interactions, such as small molecule-protein, protein. Monolith NT.115 Series | Product Information 3 MicroScale Thermophoresis A powerful technique MicroScale Thermophoresis (MST) is a powerful technique for quantifying ELRPROHFXODU LQWHUDFWLRQV %\ FRPELQLQJ WKH SUHFLVLRQ RI ÀXRUHVFHQFH GHWHFWLRQ ZLWK WKH ÀH[LELOLW\ DQG VHQVLWLYLW\ RI WKHUPRSKRUHVLV 06

Thermophoresis of DNA - NanoTemper Technologie

Monolith NT.115 is NanoTemper´s MST Instrument for basic research and pharmaceutical applications. Technology. Microscale Thermophoresis is a powerful new technology, and easy to handle. It detects changes in the hydration shell of molecules and is ideal for measuring enzyme activity and biomolecule interactions under close-to-native conditions: immobilization-free and in complex biological. Besides the acquisition of pharmacokinetic parameters of antisense oligonucleotide microRNA (miRNA) inhibitors, such as measuring in vivo concentration, their pharmacodynamic characteristics are also of interest. An emerging and straightforward method for studying molecular interactions is microscale thermophoresis (MST). This technique makes it possible to study interactions between miRNAs.

Our Equipment

Microscale Thermophoresis - a technology by NanoTempe

Monolith NT.115, includes control and analysis software: NanoTemper Technologies: MO-G008: Instrument for microscal thermophoresis: His-Tag Labeling Kit RED-Tris-NTA: NanoTemper Technologies: MO-L008: RED-tris-NTA dye for MST: Standard capillaries: NanoTemper Technologies: MO-K022: capillaries for MST: HisTrap HP, 5 mL : GE Healthcare: 17524801: IMAC column: HisPur Ni-NTA resin, 100 mL: Thermo. MicroScale Thermophoresis (MST Service) is a biophysical technique that measures the strength of interaction (affinity) between two molecules by detecting variations in fluorescence signal as a result of an IR-laser induced temperature change.The range of the variation in the fluorescence signal correlates with the binding of a ligand to the fluorescent target

Herein we request NSERC RTI funds for the purchase of an innovative instrument, Monolith NT, that uses microscale thermophoresis to measure the affinities between a fluorescent (or intrinsic Trp Fluorescent) ligand and an analyte. Nanotemper technologies created the Monolith NT detector to monitor the change in migration rate of a fluorescent probe upon interaction with an additional molecule. Realizing the shortcomings of current protein research techniques, they came up with a method, MicroScale Thermophoresis that was enormously sensitive, and would bring researchers one step closer to developing better drugs faster. Now, NanoTemper has over 120 employees in 10 offices around the world. Based on their initial idea, Stefan and Philipp invented the Monolith using the MicroScale. Determined by Microscale Thermophoresis Determined by Microscale Thermophoresis 8058 El Rio St., Houston, TX 77054 • basepairbio.com • info@basepairbio.com Figure 1 NanoTemper Technologies GmbH Monolith NT series of : instruments are based on detection of fluorescent dye (Monolith

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